7N52

Structure of a bacterial gasdermin from Runella zeae

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	80
Detector technology	PIXEL
Collection date	2020-10-09
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97911
Spacegroup name	P 1 21 1
Unit cell lengths	69.290, 103.852, 78.992
Unit cell angles	90.00, 111.54, 90.00

Refinement procedure

Resolution	42.400 - 2.900
R-factor	0.2407
Rwork	0.237
R-free	0.27950
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.581
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.400	3.070
High resolution limit [Å]	2.900	2.900
Rpim	0.060	0.431
Number of reflections	23133	3645
<I/σ(I)>	12.7	3.2
Completeness [%]	99.5
Redundancy	26.5
CC(1/2)	0.998	0.941

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.6	291	150 mM DL-malic acid (pH 7.6), 16% PEG-3350, and 10% Silver Bullets A1 solution (1,5-Naphthalenedisulfonic acid disodium salt, 2,5-Pyridinedicarboxylic acid, 3,5-Dinitrosalicylic acid, HEPES-NaOH pH 6.8) (Hampton)