7N51
Structure of a bacterial gasdermin from Vitiosangium sp.
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2020-09-26 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97911 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 87.383, 87.383, 141.969 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.760 - 1.670 |
| R-factor | 0.1945 |
| Rwork | 0.194 |
| R-free | 0.21790 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.830 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX (1.18.2_3874) |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.190 | 1.710 |
| High resolution limit [Å] | 1.670 | 1.680 |
| Rpim | 0.011 | 0.740 |
| Number of reflections | 37407 | 1876 |
| <I/σ(I)> | 28 | 0.9 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 39.4 | |
| CC(1/2) | 0.999 | 0.602 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 291 | 90 mM HEPES-KOH (pH 7.5), 1.2 M tri-sodium citrate, and 10% glycerol |
