7N3Z
Crystal Structure of Saccharomyces cerevisiae Apn2 Catalytic Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 105 |
| Detector technology | CCD |
| Collection date | 2014-07-11 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 171.412, 46.948, 51.532 |
| Unit cell angles | 90.00, 104.21, 90.00 |
Refinement procedure
| Resolution | 36.670 - 1.990 |
| R-factor | 0.1644 |
| Rwork | 0.163 |
| R-free | 0.19780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3g0r |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.915 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.670 | 2.060 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Rmeas | 0.107 | 0.548 |
| Number of reflections | 26508 | 2254 |
| <I/σ(I)> | 14.42 | 1.99 |
| Completeness [%] | 97.6 | 83.2 |
| Redundancy | 3.5 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.1 M Tris pH 8.5, 25% PEG 6000 |
