7N3V
Crystal structure of Mycobacterium smegmatis LmcA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-09-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 |
| Unit cell lengths | 34.540, 57.540, 73.812 |
| Unit cell angles | 102.57, 90.17, 100.23 |
Refinement procedure
| Resolution | 18.450 - 1.830 |
| R-factor | 0.18 |
| Rwork | 0.179 |
| R-free | 0.21300 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.030 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | Auto-Rickshaw |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.870 |
| High resolution limit [Å] | 1.830 | 1.830 |
| Rmerge | 0.074 | 0.523 |
| Rpim | 0.051 | 0.362 |
| Number of reflections | 46866 | 2577 |
| <I/σ(I)> | 12.1 | 2.9 |
| Completeness [%] | 90.7 | 86 |
| Redundancy | 5.9 | 5.5 |
| CC(1/2) | 0.998 | 0.762 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 7 | 298 | 2.2 M ammonium sulfate, 50 mM Tris, pH 7.0 |
