7N3C
Crystal Structure of Human Fab S24-202 in the complex with the N-terminal Domain of Nucleocapsid protein from SARS CoV-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-09-06 |
| Detector | DECTRIS PILATUS3 X 6M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 72.622, 64.020, 89.209 |
| Unit cell angles | 90.00, 91.09, 90.00 |
Refinement procedure
| Resolution | 42.510 - 1.820 |
| R-factor | 0.181 |
| Rwork | 0.179 |
| R-free | 0.20970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDBIDs 6IEB 6vyo |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.099 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.850 |
| High resolution limit [Å] | 1.820 | 1.820 |
| Rmerge | 0.172 | 0.911 |
| Number of reflections | 73169 | 3464 |
| <I/σ(I)> | 14.1 | 1.1 |
| Completeness [%] | 99.5 | 96.2 |
| Redundancy | 5.4 | 2.5 |
| CC(1/2) | 0.969 | 0.465 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 0.2 M Potassium iodide, 20% w/v Polyethylene glycol 3,350 |
