7N3B

Crystal structure of aged 9-site deamidated variant of human gamma(S)-crystallin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-23
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9763
Spacegroup name	P 2 21 21
Unit cell lengths	48.650, 75.838, 93.374
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	58.870 - 2.090
R-factor	0.191
Rwork	0.188
R-free	0.23960
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6fd8
RMSD bond length	0.007
RMSD bond angle	0.943
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	58.870	2.170
High resolution limit [Å]	2.090	2.090
Number of reflections	20969	2008
<I/σ(I)>	13.98
Completeness [%]	99.7
Redundancy	12.9
CC(1/2)	0.998	0.674

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M sodium acetate pH 5.45, 0.1 M ammonium sulfate, 21% PEG 3350