7N3A

Crystal structure of 9-site deamidated variant of human gamma(S)-crystallin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-08-25
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9762
Spacegroup name	P 1 21 1
Unit cell lengths	29.770, 57.710, 44.950
Unit cell angles	90.00, 103.33, 90.00

Refinement procedure

Resolution	43.740 - 1.500
R-factor	0.1968
Rwork	0.195
R-free	0.22370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6fd8
RMSD bond length	0.008
RMSD bond angle	0.987
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.740	1.550
High resolution limit [Å]	1.500	1.500
Number of reflections	23517	2292
<I/σ(I)>	13.85
Completeness [%]	98.4
Redundancy	6.8
CC(1/2)	0.999	0.734

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M sodium acetate pH 5.45, 21% PEG 3350