7N36
Crystal structure of wild-type human gamma(S)-crystallin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P14 (MX2) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-08-25 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.840, 60.630, 56.030 |
| Unit cell angles | 90.00, 117.46, 90.00 |
Refinement procedure
| Resolution | 46.890 - 2.000 |
| R-factor | 0.1907 |
| Rwork | 0.189 |
| R-free | 0.21990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6fd8 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.877 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.890 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 20807 | 2056 |
| <I/σ(I)> | 16.29 | |
| Completeness [%] | 97.4 | |
| Redundancy | 7 | |
| CC(1/2) | 0.999 | 0.782 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | Morpheus condition G5 (0.1 M Carboxylic Acids Mix, 0.1 M Buffer System 2 pH 7.5, 30 % v/v Precipitant Mix 1) |
