7N0R
Structure of the SARS-CoV-2 N protein RNA-binding domain bound to single-domain antibody C2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-02-11 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97918 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 46.668, 77.120, 71.932 |
Unit cell angles | 90.00, 95.14, 90.00 |
Refinement procedure
Resolution | 52.490 - 1.420 |
R-factor | 0.1417 |
Rwork | 0.141 |
R-free | 0.16620 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7cdz |
RMSD bond length | 0.008 |
RMSD bond angle | 0.964 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 77.120 | 1.440 |
High resolution limit [Å] | 1.420 | 1.420 |
Rmerge | 0.037 | 0.257 |
Rpim | 0.016 | 0.121 |
Number of reflections | 95122 | 4475 |
<I/σ(I)> | 25.7 | 4.5 |
Completeness [%] | 99.1 | 94.4 |
Redundancy | 6.7 | 6.2 |
CC(1/2) | 0.999 | 0.976 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M Tris-HCl pH 8.5, 0.2 M LiSO4, and 20% PEG 4000 |