7N0R

Structure of the SARS-CoV-2 N protein RNA-binding domain bound to single-domain antibody C2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-11
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 1 21 1
Unit cell lengths	46.668, 77.120, 71.932
Unit cell angles	90.00, 95.14, 90.00

Refinement procedure

Resolution	52.490 - 1.420
R-factor	0.1417
Rwork	0.141
R-free	0.16620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7cdz
RMSD bond length	0.008
RMSD bond angle	0.964
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	77.120	1.440
High resolution limit [Å]	1.420	1.420
Rmerge	0.037	0.257
Rpim	0.016	0.121
Number of reflections	95122	4475
<I/σ(I)>	25.7	4.5
Completeness [%]	99.1	94.4
Redundancy	6.7	6.2
CC(1/2)	0.999	0.976

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M Tris-HCl pH 8.5, 0.2 M LiSO4, and 20% PEG 4000