7N0J
Structure of YebY from E. coli K12
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 77 |
| Detector technology | PIXEL |
| Collection date | 2019-10-30 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.9786 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 96.145, 53.199, 132.363 |
| Unit cell angles | 90.00, 102.25, 90.00 |
Refinement procedure
| Resolution | 34.670 - 1.880 |
| R-factor | 0.2362 |
| Rwork | 0.235 |
| R-free | 0.28210 |
| Structure solution method | SAD |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 34.670 | 34.670 | 1.920 |
| High resolution limit [Å] | 1.880 | 8.390 | 1.880 |
| Rmerge | 0.100 | 0.034 | 1.695 |
| Rmeas | 0.119 | 0.040 | 2.168 |
| Total number of observations | 645748 | ||
| Number of reflections | 188643 | 2323 | 7652 |
| <I/σ(I)> | 6.97 | 27.72 | 0.44 |
| Completeness [%] | 89.8 | 98.8 | 49 |
| Redundancy | 3.423 | 3.701 | 2.575 |
| CC(1/2) | 0.997 | 0.998 | 0.261 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M ammonium sulfate, 0.1 M Bis-Tris-HCl (pH 6.5), and 25% (w/v) PEG 3350 |
