7N0I
Structure of the SARS-CoV-2 N protein C-terminal domain bound to single-domain antibody E2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-05-14 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 75.846, 131.557, 140.048 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.690 - 2.200 |
| R-factor | 0.241 |
| Rwork | 0.239 |
| R-free | 0.27140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6wzq |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.683 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.690 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.089 | 5.417 |
| Rpim | 0.038 | 2.282 |
| Number of reflections | 129860 | 6374 |
| <I/σ(I)> | 8.1 | 0.4 |
| Completeness [%] | 99.7 | |
| Redundancy | 6.5 | |
| CC(1/2) | 0.999 | 0.344 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20 mM HEPES pH 7.0, 200 mM NaCl, 5 mM MgCl2, 1 mM TCEP |
