7N0E
Co-complex of the histidine kinase region of RetS and the dimerization and histidine phosphotransfer domain of GacS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CMOS |
Collection date | 2019-03-20 |
Detector | RDI CMOS_8M |
Wavelength(s) | 0.97625 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 103.360, 103.360, 61.960 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.690 - 2.300 |
R-factor | 0.2486 |
Rwork | 0.247 |
R-free | 0.26620 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6dk7 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.18_3855) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 44.760 | 44.760 | 2.370 |
High resolution limit [Å] | 2.290 | 8.870 | 2.290 |
Rmerge | 0.147 | 0.023 | 9.990 |
Rmeas | 0.150 | 0.024 | 14.059 |
Rpim | 0.033 | 0.005 | 3.045 |
Total number of observations | 369869 | ||
Number of reflections | 17507 | 341 | 1708 |
<I/σ(I)> | 16.9 | ||
Completeness [%] | 100.0 | 99.2 | 100 |
Redundancy | 21.1 | 19.5 | 21.1 |
CC(1/2) | 1.000 | 1.000 | 0.397 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295.15 | 0.2 M lithium sulfate, 0.1 M tris pH 8.5, 30% PEG 3350 |