7N02
X-ray crystallographic structure model of Lactococcus lactis prolidase mutant D36S
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08B1-1 |
Synchrotron site | CLSI |
Beamline | 08B1-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-01-31 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1.5215 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 83.120, 87.270, 119.400 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.635 - 2.350 |
R-factor | 0.2152 |
Rwork | 0.212 |
R-free | 0.26990 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4zng |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.640 | 2.470 |
High resolution limit [Å] | 2.350 | 2.350 |
Number of reflections | 36748 | 3603 |
<I/σ(I)> | 11.8 | |
Completeness [%] | 99.7 | 99.5 |
Redundancy | 6.1 | 6.48 |
CC(1/2) | 0.998 | 0.517 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277.15 | 0.1 M calcium acetate, 0.1 M Bis-Tris propane, 12-15% w/v PEG4000 |