7MYX
Crystal structure of the PH domain (R86A) of Akt1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-02-14 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.979180 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 84.786, 33.627, 42.352 |
Unit cell angles | 90.00, 119.33, 90.00 |
Refinement procedure
Resolution | 36.960 - 1.390 |
R-factor | 0.1782 |
Rwork | 0.175 |
R-free | 0.20410 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1unp |
RMSD bond length | 0.006 |
RMSD bond angle | 0.791 |
Data reduction software | XDS (1.19.1_4122+SVN) |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.1_4122+SVN) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.960 | 1.440 |
High resolution limit [Å] | 1.390 | 1.390 |
Rmerge | 0.041 | 0.112 |
Rmeas | 0.049 | 0.134 |
Rpim | 0.026 | 0.074 |
Number of reflections | 20816 | 1987 |
<I/σ(I)> | 32.04 | |
Completeness [%] | 97.2 | 95.07 |
Redundancy | 3.3 | 3.1 |
CC(1/2) | 0.996 | 0.982 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | 298 | 1.28 M Sodium Citrate, 0.1 M Hepes pH 7.5, 0.01 M Praseodymium(III) Acetate |