7MYX

Crystal structure of the PH domain (R86A) of Akt1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-14
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979180
Spacegroup name	C 1 2 1
Unit cell lengths	84.786, 33.627, 42.352
Unit cell angles	90.00, 119.33, 90.00

Refinement procedure

Resolution	36.960 - 1.390
R-factor	0.1782
Rwork	0.175
R-free	0.20410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1unp
RMSD bond length	0.006
RMSD bond angle	0.791
Data reduction software	XDS (1.19.1_4122+SVN)
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122+SVN)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.960	1.440
High resolution limit [Å]	1.390	1.390
Rmerge	0.041	0.112
Rmeas	0.049	0.134
Rpim	0.026	0.074
Number of reflections	20816	1987
<I/σ(I)>	32.04
Completeness [%]	97.2	95.07
Redundancy	3.3	3.1
CC(1/2)	0.996	0.982

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	298	1.28 M Sodium Citrate, 0.1 M Hepes pH 7.5, 0.01 M Praseodymium(III) Acetate