7MYV

Plasmodium falciparum HAD5/PMM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-30
Detector	DECTRIS PILATUS3 X 6M
Wavelength(s)	0.98
Spacegroup name	P 65 2 2
Unit cell lengths	161.255, 161.255, 109.738
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.310 - 3.510
R-factor	0.2593
Rwork	0.254
R-free	0.30710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6cfr
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.316	3.560
High resolution limit [Å]	3.500	3.500
Number of reflections	11030	543
<I/σ(I)>	18.5	2.7
Completeness [%]	99.9	100
Redundancy	32.1	30.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	2ul drops containing 1:1 mixture of protein (6mg/mL) and crystallization buffer (0.1M bis -tris-propane pH 6.5, 0.1 M calcium acetate, 16% (w/v) PEG 8000, and 2% (v/v) benzamidine hydrochloride)