7MYT
Crystal Structure of HPV L1-directed B25.M05 Fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-11-25 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 |
| Unit cell lengths | 52.949, 64.475, 72.792 |
| Unit cell angles | 94.41, 97.13, 93.31 |
Refinement procedure
| Resolution | 45.980 - 2.100 |
| R-factor | 0.1753 |
| Rwork | 0.173 |
| R-free | 0.22280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6xqw |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.286 |
| Data reduction software | HKL-3000 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 52264 | 2588 |
| <I/σ(I)> | 10.5 | |
| Completeness [%] | 94.6 | |
| Redundancy | 4.9 | |
| CC(1/2) | 0.980 | 0.840 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 3.35% v/v PEG 400, 1.34 M (NH4)3Citrate pH 7.5 |
