7MYQ

Crystal structure of a tRNA (guanine-N1)-methyltransferase from Acinetobacter baumannii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2021-02-25
Detector	RAYONIX MX-300
Wavelength(s)	0.9787
Spacegroup name	P 41
Unit cell lengths	66.250, 66.250, 133.010
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.850 - 2.550
R-factor	0.197
Rwork	0.193
R-free	0.22930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ual
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (dev-4224-000)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.850	46.850	2.620
High resolution limit [Å]	2.550	11.400	2.550
Rmerge	0.047	0.024	0.584
Rmeas	0.051	0.026	0.636
Total number of observations	119128
Number of reflections	18708	206	1358
<I/σ(I)>	24.4	59.04	3.52
Completeness [%]	99.9	92.4	99.9
Redundancy	6.368	5.738	6.403
CC(1/2)	0.999	0.999	0.932

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	287	AcbaC.00054.a.B1.PW38905 at 27 mg/mL against Morpheus condition D3 with 10% PEG 4000, 20% glycerol, 0.1 M MES/imidazole pH 6.5, 0.02 M each 1,6-hexanoediol, 1-butanol, 1,2-propanediol, 2-propanol, 1,4-butanediol, 1,3-propanediol, direct cryo, unique puck ID okj1-6, 319637d3