7MY6
Se-CrtE C-term His-tag with IPP added
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-03-21 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9537 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 45.423, 144.627, 48.066 |
Unit cell angles | 90.00, 95.25, 90.00 |
Refinement procedure
Resolution | 45.482 - 2.020 |
Rwork | 0.180 |
R-free | 0.20880 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7mxz |
RMSD bond length | 0.008 |
RMSD bond angle | 1.407 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.210 | 1.990 |
High resolution limit [Å] | 1.940 | 1.940 |
Rmerge | 0.345 | 2.327 |
Rpim | 0.113 | 0.781 |
Number of reflections | 45579 | 3076 |
<I/σ(I)> | 5.7 | 1.1 |
Completeness [%] | 99.9 | |
Redundancy | 10 | 9.7 |
CC(1/2) | 0.986 | 0.392 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9.5 | 293 | 200 nL of protein at 10 mg/mL was added to 200 nL of reservoir consisting of 90 mM CHES pH 9.5, 180 mM magnesium sulfate, 100 mM trisodium citrate, 22.5% (w/v) PEG 1500 |