7MY0
Sy-CrtE IPP structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-03-21 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 61.430, 73.643, 132.229 |
| Unit cell angles | 90.00, 95.21, 90.00 |
Refinement procedure
| Resolution | 43.930 - 1.370 |
| Rwork | 0.128 |
| R-free | 0.16000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7mxz |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.867 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.060 | 1.390 |
| High resolution limit [Å] | 1.370 | 1.370 |
| Rmerge | 0.186 | 2.746 |
| Rpim | 0.042 | 0.720 |
| Number of reflections | 122978 | 6040 |
| <I/σ(I)> | 9.2 | 1.1 |
| Completeness [%] | 100.0 | |
| Redundancy | 20.1 | |
| CC(1/2) | 0.998 | 0.520 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | The protein at 10 mg/mL was set up in 200 plus 200 nL drops with the reservoir as 20% (w/v) PEG 3350 and 0.2 M magnesium acetate |
