7MXT
Crystal structure of human exonuclease 1 Exo1 (WT) in complex with 5' recessed-end DNA (cmr)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 12.3.1 |
Synchrotron site | ALS |
Beamline | 12.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-10-23 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 72.857, 72.857, 181.382 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.498 - 3.048 |
R-factor | 0.2151 |
Rwork | 0.211 |
R-free | 0.25970 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5v05 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.853 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.100 |
High resolution limit [Å] | 3.048 | 3.050 |
Rmerge | 0.077 | 0.682 |
Number of reflections | 9928 | 471 |
<I/σ(I)> | 27.54 | |
Completeness [%] | 99.4 | |
Redundancy | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 290 | 100 mM sodium acetate, pH 7.0; 10 mM KCl; 2-4% PEG 4000 |