7MX4

CD1c with antigen analogue 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-09
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9322
Spacegroup name	P 21 21 21
Unit cell lengths	55.419, 71.737, 100.761
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.560 - 1.730
R-factor	0.2084
Rwork	0.207
R-free	0.23880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ov6
RMSD bond length	0.007
RMSD bond angle	1.069
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX (1.18.2_3874-000)
Refinement software	PHENIX (1.18.2_3874-000)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.560	1.792
High resolution limit [Å]	1.730	1.730
Rmerge	0.020	0.331
Rmeas	0.028	0.467
Rpim	0.020	0.331
Number of reflections	42680	4208
<I/σ(I)>	11.61	1.71
Completeness [%]	99.8	99.33
Redundancy	2	2
CC(1/2)	1.000	0.796

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9.4	293	100 mM CHES, 1.05 M sodium citrate, 25 mM triglycine