7MWY
Structure of the drosophila STING cyclic dinucleotide binding domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2021-04-01 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 135.199, 135.199, 60.035 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.890 - 1.840 |
| R-factor | 0.18 |
| Rwork | 0.179 |
| R-free | 0.19370 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | SAD-phased model of the selenomethionine derivative |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.230 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.910 | 1.880 |
| High resolution limit [Å] | 1.840 | 1.840 |
| Rpim | 0.035 | 0.656 |
| Number of reflections | 54801 | 3340 |
| <I/σ(I)> | 11.2 | 1.1 |
| Completeness [%] | 99.7 | 98.6 |
| Redundancy | 5.8 | 5.8 |
| CC(1/2) | 0.999 | 0.435 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.2 M sodium citrate, 0.1 M Tris-HCl, 22% PEG-3350 |
