7MWS
Crystal structure of tamarin CD81 large extracellular loop
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-13 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.979 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 113.263, 113.263, 113.263 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.040 - 1.800 |
| R-factor | 0.2083 |
| Rwork | 0.207 |
| R-free | 0.23150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g8q |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.379 |
| Data reduction software | MOSFLM (7.2.2) |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.040 | 1.870 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 22547 | 1651 |
| <I/σ(I)> | 14.2 | |
| Completeness [%] | 100.0 | |
| Redundancy | 40.1 | |
| CC(1/2) | 0.990 | 0.670 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1M HEPES pH 7.5, and 55% PEG 200 |
