7MWI

Crystal structure of human BAZ2A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2014-02-20
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979237
Spacegroup name	P 61 2 2
Unit cell lengths	47.860, 47.860, 238.861
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.450 - 1.800
R-factor	0.1857
Rwork	0.184
R-free	0.21270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3c2i
RMSD bond length	0.019
RMSD bond angle	1.933
Data scaling software	Aimless (0.2.17)
Phasing software	PHASER
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.770	47.770	1.780
High resolution limit [Å]	1.750	9.090	1.750
Rmerge	0.064	0.025	1.140
Rmeas	0.067	0.026	1.194
Rpim	0.015	0.007	0.258
Number of reflections	17590	185	951
<I/σ(I)>	34.5
Completeness [%]	100.0	98.3	100
Redundancy	20.1	12.1	20.9
CC(1/2)	1.000	1.000	0.939

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	291	2.0M Sodium Formate, 0.1M Tris pH 8.5