7MWC
ERAP1 binds peptide C-terminus of a LPF sequence (AAAAFKARKF)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-03-01 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 |
Unit cell lengths | 57.030, 66.680, 121.710 |
Unit cell angles | 90.07, 101.81, 90.05 |
Refinement procedure
Resolution | 55.120 - 3.000 |
R-factor | 0.244 |
Rwork | 0.238 |
R-free | 0.29800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3rjo |
RMSD bond length | 0.011 |
RMSD bond angle | 1.627 |
Data reduction software | iMOSFLM |
Data scaling software | pointless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 58.200 | 3.200 |
High resolution limit [Å] | 3.000 | 3.000 |
Number of reflections | 31557 | 4503 |
<I/σ(I)> | 7.6 | 2.4 |
Completeness [%] | 90.0 | 87.5 |
Redundancy | 2.25 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 100mM Tris, pH 8.5, 12% w/v PEG8000 |