7MW1

Crystal structure of the Homo sapiens NUP93-NUP53 complex (NUP93 residues 174-819; NUP53 residues 84-150)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-07
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 1 21 1
Unit cell lengths	67.694, 181.439, 78.945
Unit cell angles	90.00, 94.03, 90.00

Refinement procedure

Resolution	33.760 - 3.400
R-factor	0.2328
Rwork	0.229
R-free	0.27630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	to be deposited
RMSD bond length	0.003
RMSD bond angle	0.564
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	3.521
High resolution limit [Å]	3.400	3.400
Rmerge	0.121	2.094
Rmeas	0.131	2.258
Rpim	0.049	0.837
Number of reflections	25795	2574
<I/σ(I)>	10.1	0.9
Completeness [%]	98.5	99.3
Redundancy	7	7.2
CC(1/2)	1.000	0.497

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	294	0.075 M TRIS pH 8.5, 17% (w/v) PEG 20000