7MVX

Crystal structure of the Chaetomium thermophilum Nup188-Nic96 complex (Nup188 residues 1-1858; Nic96 residues 240-301)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97944
Spacegroup name	H 3 2
Unit cell lengths	302.648, 302.648, 152.675
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.930 - 4.350
R-factor	0.2745
Rwork	0.272
R-free	0.31670
Structure solution method	SAD
Data reduction software	DIALS
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	4.860
High resolution limit [Å]	4.350	4.350
Rmerge	0.247	2.429
Rmeas	0.253	2.493
Rpim	0.057	0.559
Number of reflections	17578	4981
<I/σ(I)>	11.3
Completeness [%]	99.5	100
Redundancy	19.6	19.7
CC(1/2)	1.000	0.777

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	294	0.05 M HEPES pH 7.5, 6.5 % (w/v) PEG 20000