7MVW

Crystal structure of Chaetomium thermophilum Nup188 NTD (residues 1-1134)

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-24
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97947, 0.97963, 0.96108
Spacegroup name	P 64
Unit cell lengths	173.541, 173.541, 111.124
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.390 - 2.760
R-factor	0.2243
Rwork	0.223
R-free	0.25420
Structure solution method	MAD
RMSD bond length	0.002
RMSD bond angle	0.476
Data reduction software	XDS
Data scaling software	XDS
Phasing software	SHARP
Refinement software	PHENIX (1.19.1_4122+SVN)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.000	2.859
High resolution limit [Å]	2.760	2.760
Rmerge	0.162	2.103
Rmeas	0.171	2.225
Rpim	0.053	0.715
Number of reflections	48981	4880
<I/σ(I)>	13.7	1.26
Completeness [%]	99.8	99.63
Redundancy	10.4	9.5
CC(1/2)	1.000	0.570

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	294	10 % (w/v) PEG 3350, 0.1 M KSCN
1	VAPOR DIFFUSION, HANGING DROP	8	294	10 % (w/v) PEG 3350, 0.1 M KSCN