7MSK
ThuS glycosin S-glycosyltransferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2019-12-19 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 0.9786 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.833, 112.654, 90.310 |
| Unit cell angles | 90.00, 90.11, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.060 |
| R-factor | 0.2024 |
| Rwork | 0.201 |
| R-free | 0.23890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.161 |
| Data reduction software | autoPROC |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.300 | 2.090 |
| High resolution limit [Å] | 2.060 | 2.060 |
| Number of reflections | 61544 | 3042 |
| <I/σ(I)> | 16.9 | |
| Completeness [%] | 99.7 | |
| Redundancy | 6.3 | |
| CC(1/2) | 0.999 | 0.831 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 283 | 15% PEG 3350 200 mM ammonium nitrate 100 mM BisTris HCl pH=6.5 |
