7MS0
Crystal structure of native Cg10062
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-03-03 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 49.577, 49.577, 80.067 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.030 - 1.370 |
| Rwork | 0.171 |
| R-free | 0.18580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3n4g |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.791 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.17.1-3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.030 | 1.420 |
| High resolution limit [Å] | 1.370 | 1.370 |
| Rmerge | 0.059 | 0.814 |
| Rmeas | 0.061 | 0.836 |
| Rpim | 0.013 | 0.190 |
| Number of reflections | 24562 | 2401 |
| <I/σ(I)> | 40.1 | |
| Completeness [%] | 99.9 | 99.42 |
| Redundancy | 20 | |
| CC(1/2) | 0.911 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 300 | 50 mM magnesium chloride hexahydrate, 25 mM HEPES sodium, pH 7.5, 7.5% v/v PEG4000 |
