7MRL

Structure of HIV-1 matrix domain bound to human tRNALys3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-21
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	I 2 2 2
Unit cell lengths	96.717, 108.846, 146.021
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.110 - 3.150
R-factor	0.1889
Rwork	0.185
R-free	0.22310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB entries 1HIW & 1FIR
Data reduction software	MOSFLM
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.114	3.263
High resolution limit [Å]	3.150	3.150
Number of reflections	13692	13657
<I/σ(I)>	8.7	1.23
Completeness [%]	99.7	99.55
Redundancy	18.6
CC(1/2)	0.998	0.576

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	100 mM HEPES, 20% PEG500 MME, 10% PEG20000