7MRI
Crystal structure of N63T yeast iso-1-cytochrome c
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2019-06-13 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 41 |
| Unit cell lengths | 59.250, 59.250, 92.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.930 - 2.460 |
| R-factor | 0.1675 |
| Rwork | 0.166 |
| R-free | 0.20800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ycc |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.628 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.930 | 2.520 |
| High resolution limit [Å] | 2.460 | 2.460 |
| Rmerge | 0.190 | 0.730 |
| Number of reflections | 11569 | 824 |
| <I/σ(I)> | 6.5 | |
| Completeness [%] | 99.7 | |
| Redundancy | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.1 M Tris, pH 8.5, 30% w/v PEG1000 |
