7MQZ

Cytochrome c oxidase assembly factor 7

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-09-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9536
Spacegroup name	I 41
Unit cell lengths	100.010, 100.010, 50.343
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.970 - 2.390
R-factor	0.2077
Rwork	0.205
R-free	0.25480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ouv
RMSD bond length	0.010
RMSD bond angle	1.301
Data reduction software	XDS
Data scaling software	Aimless (0.7.1)
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.970	2.480
High resolution limit [Å]	2.390	2.390
Rmerge	0.093	0.730
Rmeas	0.101	0.791
Rpim	0.039	0.301
Total number of observations	68093
Number of reflections	10025	1012
<I/σ(I)>	10.5
Completeness [%]	99.7	96.9
Redundancy	6.8	6.7
CC(1/2)	0.998	0.816

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.23 M MES sodium, pH 6.72, 2.35 M ammonium sulfate, 8.2% v/v ethanol, 4% v/v pentaerythritol ethoxylate (3/4 EO/OH)