7MQW

Histidine triad protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-04
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97918
Spacegroup name	P 43 21 2
Unit cell lengths	55.149, 55.149, 205.735
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.600 - 1.550
R-factor	0.1812
Rwork	0.180
R-free	0.20640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3p0t
RMSD bond length	0.006
RMSD bond angle	0.884
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.580
High resolution limit [Å]	1.550	1.550
Rpim	0.024	0.281
Number of reflections	47498	2314
<I/σ(I)>	41.3
Completeness [%]	99.9
Redundancy	11.7
CC(1/2)	0.998	0.861

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	15.6 mg/mL (1mM) protein + 5 mM ATP in 0.2 M ammonium sulfate, 30% w/v PEG4000