7MQQ

Structure of an allelic variant of Puccinia graminis f. sp. tritici (Pgt) effector AvrSr50 (QCMJC)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-23
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	P 32 2 1
Unit cell lengths	50.732, 50.732, 91.091
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	31.620 - 1.150
R-factor	0.1626
Rwork	0.162
R-free	0.17680
Structure solution method	SAD
Starting model (for MR)	SAD AvrSr50
RMSD bond length	0.019
RMSD bond angle	1.551
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	CRANK2
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.620	1.190
High resolution limit [Å]	1.150	1.150
Rmerge	0.044	0.915
Rmeas		0.960
Rpim	0.013	0.288
Number of reflections	48941	4807
<I/σ(I)>	23.43
Completeness [%]	100.0	99.96
Redundancy	11.5
CC(1/2)	1.000	0.863

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	60% Tacsimate pH 7.0