7MQJ
Dhr1 Helicase Core
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-1 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-02-12 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.97919 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.770, 130.940, 86.080 |
| Unit cell angles | 90.00, 106.48, 90.00 |
Refinement procedure
| Resolution | 39.362 - 2.230 |
| R-factor | 0.1817 |
| Rwork | 0.179 |
| R-free | 0.22740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xau |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3211) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.370 | 2.310 |
| High resolution limit [Å] | 2.230 | 2.230 |
| Rmerge | 0.082 | |
| Rmeas | 0.097 | |
| Rpim | 0.052 | |
| Number of reflections | 42918 | 4275 |
| <I/σ(I)> | 11.89 | |
| Completeness [%] | 99.3 | 98.68 |
| Redundancy | 3.4 | |
| CC(1/2) | 0.997 | 0.784 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277.15 | PEG6000, HEPES, pH 7.0, magnesium chloride, ADP |
