7MQ7

Tetragonal Maltose Binding Protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	77
Detector technology	PIXEL
Collection date	2017-03-21
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.08724
Spacegroup name	P 43 21 2
Unit cell lengths	69.070, 69.070, 211.803
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.001 - 1.950
R-factor	0.197
Rwork	0.195
R-free	0.23470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1anf
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.001	2.010
High resolution limit [Å]	1.950	1.950
Number of reflections	35971	3070
<I/σ(I)>	16
Completeness [%]	95.0
Redundancy	8.3
CC(1/2)	0.992	0.948

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		298	2.4 M ammonium sulfate, 0.1 M sodium acetate pH 4.5