7MQ6

Tetragonal Maltose Binding Protein in the presence of gold

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	77
Detector technology	PIXEL
Collection date	2017-03-21
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.92017
Spacegroup name	P 43 21 2
Unit cell lengths	68.327, 68.327, 210.382
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.581 - 2.372
R-factor	0.2122
Rwork	0.210
R-free	0.24870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1anf
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.450
High resolution limit [Å]	2.370	2.370
Number of reflections	20852	2772
<I/σ(I)>	15.8
Completeness [%]	98.8
Redundancy	5.2
CC(1/2)	0.990	0.972

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		298	2.4 M ammonium sulfate, 0.1 M sodium acetate pH 4.5