7MQ5
Crystal Structure of Putative Universal Stress Protein from Pseudomonas aeruginosa UCBPP-PA14
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-11-14 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97856 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 43.069, 67.866, 48.527 |
Unit cell angles | 90.00, 110.89, 90.00 |
Refinement procedure
Resolution | 27.170 - 1.250 |
R-factor | 0.1297 |
Rwork | 0.128 |
R-free | 0.15660 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3mt0 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.360 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.270 |
High resolution limit [Å] | 1.250 | 1.250 |
Rmerge | 0.055 | 0.729 |
Rmeas | 0.063 | 0.868 |
Rpim | 0.030 | 0.464 |
Number of reflections | 72361 | 3642 |
<I/σ(I)> | 23.3 | 1.73 |
Completeness [%] | 100.0 | 99.8 |
Redundancy | 4.2 | 3.4 |
CC(1/2) | 0.508 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 292 | Protein: 16.2 mg/ml, 0.5 Sodium chloride, 0.01M Tris pH 8.3; Screen: PACT (C11), 0.2M Calcium chloride, 0.1M HEPES pH 7.0, 20% (w/v) PEG 6000 |