7MQ5

Crystal Structure of Putative Universal Stress Protein from Pseudomonas aeruginosa UCBPP-PA14

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2019-11-14
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97856
Spacegroup name	P 1 21 1
Unit cell lengths	43.069, 67.866, 48.527
Unit cell angles	90.00, 110.89, 90.00

Refinement procedure

Resolution	27.170 - 1.250
R-factor	0.1297
Rwork	0.128
R-free	0.15660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3mt0
RMSD bond length	0.006
RMSD bond angle	1.360
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.270
High resolution limit [Å]	1.250	1.250
Rmerge	0.055	0.729
Rmeas	0.063	0.868
Rpim	0.030	0.464
Number of reflections	72361	3642
<I/σ(I)>	23.3	1.73
Completeness [%]	100.0	99.8
Redundancy	4.2	3.4
CC(1/2)		0.508

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	292	Protein: 16.2 mg/ml, 0.5 Sodium chloride, 0.01M Tris pH 8.3; Screen: PACT (C11), 0.2M Calcium chloride, 0.1M HEPES pH 7.0, 20% (w/v) PEG 6000