7MPL
Bartonella henselae NrnC bound to pGG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-20 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.160, 128.263, 129.281 |
| Unit cell angles | 90.00, 94.68, 90.00 |
Refinement procedure
| Resolution | 64.390 - 1.800 |
| R-factor | 0.1519 |
| Rwork | 0.151 |
| R-free | 0.17110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yt3 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.765 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.390 | 1.864 |
| High resolution limit [Å] | 1.799 | 1.799 |
| Number of reflections | 212143 | 20933 |
| <I/σ(I)> | 17.5 | |
| Completeness [%] | 99.2 | 98.12 |
| Redundancy | 4.2 | |
| CC(1/2) | 0.999 | 0.602 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 294 | 0.1 M Succinic acid; 10% PEG 3,350; 20% xylitol |
