7MPC
Structure of SsoPTP bound to vanadate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 84 |
Detector technology | PIXEL |
Collection date | 2019-06-27 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97946 |
Spacegroup name | P 63 |
Unit cell lengths | 84.582, 84.582, 42.346 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 42.290 - 1.750 |
R-factor | 0.1681 |
Rwork | 0.167 |
R-free | 0.20290 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2i6i |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER (2.8.0) |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.810 |
High resolution limit [Å] | 1.750 | 3.770 | 1.750 |
Rmerge | 0.186 | 0.146 | 0.562 |
Rmeas | 0.191 | 0.150 | 0.595 |
Rpim | 0.043 | 0.034 | 0.189 |
Number of reflections | 17577 | 1834 | 1712 |
<I/σ(I)> | 17.4 | ||
Completeness [%] | 99.8 | 98.6 | |
Redundancy | 17.5 | 8.6 | |
CC(1/2) | 0.993 | 0.759 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.1M Hepes, 0.001 M zinc chloride, 20% PEG 6000 |