7MP8

Crystal structure of the cytosolic domain of Tribolium castaneum PINK1 in the non-phosphorylated state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-03-14
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97949
Spacegroup name	P 61 2 2
Unit cell lengths	52.914, 52.914, 545.362
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.820 - 3.000
R-factor	0.2564
Rwork	0.254
R-free	0.30620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5yj9
RMSD bond length	0.004
RMSD bond angle	0.668
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.580	3.180
High resolution limit [Å]	3.000	3.000
Rmerge	0.737	3.613
Number of reflections	10223	1589
<I/σ(I)>	6.1	1.1
Completeness [%]	100.0	100
Redundancy	16.4	12.6
CC(1/2)	0.989	0.627

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		288	0.1M HEPES (pH 7.0), 0.15M (NH4)2 SO4, 20% PEG 4K. Temp = 295K