7MNV

Crystal Structure of the ZnF8 of Nucleoporin NUP358/RanBP2 in complex with Ran-GDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-06
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.2825
Spacegroup name	P 21 21 2
Unit cell lengths	60.470, 80.470, 58.320
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.300 - 1.800
R-factor	0.1606
Rwork	0.159
R-free	0.18550
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	xia2
Phasing software	AutoSol
Refinement software	PHENIX (1.18.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.050	29.050	1.860
High resolution limit [Å]	1.800	3.880	1.800
Rmeas	0.119	0.053	1.896
Rpim	0.033	0.015	0.533
Total number of observations	341183	33750	31307
Number of reflections	26934	2885	2561
<I/σ(I)>	14	40	1.2
Completeness [%]	99.6	100	96.4
Redundancy	12.7	11.7	12.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.7	294	19% (w/v) PEG 3350; 0.1 M Bis-Tris