7MNR

Crystal Structure of the ZnF3 of Nucleoporin NUP358/RanBP2 in complex with Ran-GDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-01
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.2824
Spacegroup name	P 21 21 2
Unit cell lengths	59.570, 80.200, 54.830
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.440 - 1.800
R-factor	0.1612
Rwork	0.160
R-free	0.19580
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	xia2
Phasing software	AutoSol
Refinement software	PHENIX (1.18.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	28.440	28.440	1.860
High resolution limit [Å]	1.800	3.880	1.800
Rmeas	0.097	0.045	1.245
Rpim	0.038	0.018	0.590
Total number of observations	153861	16691	9866
Number of reflections	24915	2682	2354
<I/σ(I)>	13.7	37.2	1.3
Completeness [%]	99.7	100	95.8
Redundancy	6.2	6.2	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	294	19% (w/v) PEG 3350; 0.1 M Bis-Tris