7MNO

Crystal structure of the N-terminal domain of NUP358/RanBP2 (residues 1-752) I656V mutant in complex with Fab fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-26
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0332
Spacegroup name	P 65 2 2
Unit cell lengths	161.565, 161.565, 645.405
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.050 - 6.730
R-factor	0.2078
Rwork	0.205
R-free	0.25410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ga0 5cws
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	PHENIX (1.17.1)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.050	30.050	7.520
High resolution limit [Å]	6.730	15.050	6.730
Rmerge	0.554	0.165	3.215
Rmeas	0.562	0.167	3.259
Rpim	0.091	0.029	0.529
Total number of observations	348970	27201	95598
Number of reflections	9487	862	2593
<I/σ(I)>	8.5	19.4	1.6
Completeness [%]	98.6	87.5	98.9
Redundancy	36.8	31.6	36.9
CC(1/2)	0.993	0.998	0.621

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	294	3.5 % (w/v) PEG 4,000; 0.15 M sodium acetate; 0.1 M sodium citrate