7MNN

Crystal structure of the N-terminal domain of NUP358/RanBP2 (residues 1-752) T653I mutant in complex with Fab fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-26
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 65 2 2
Unit cell lengths	161.537, 161.537, 647.375
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 6.700
R-factor	0.2197
Rwork	0.217
R-free	0.26340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ga0 5cws
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	PHENIX (1.17.1)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	7.490
High resolution limit [Å]	6.700	14.980	6.700
Rmerge	0.779	0.184	6.378
Rmeas	0.783	0.185	6.409
Rpim	0.077	0.019	0.621
Total number of observations	955034	75729	275234
Number of reflections	9674	873	2677
<I/σ(I)>	11	30.6	1.5
Completeness [%]	98.9	87.5	100
Redundancy	98.7	86.7	102.8
CC(1/2)	0.996	0.999	0.675

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	294	4 (w/v) % PEG 4,000; 0.15 M sodium acetate; 0.1 M sodium citrate