7MNL

Crystal structure of the N-terminal domain of NUP358/RanBP2 (residues 1-752) in complex with Fab fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-09
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0331
Spacegroup name	P 65 2 2
Unit cell lengths	161.420, 161.420, 644.723
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.970 - 3.950
R-factor	0.1946
Rwork	0.192
R-free	0.24140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ga0 5cws
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17.1)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	19.970	19.970	4.100
High resolution limit [Å]	3.950	14.770	3.950
Rmerge	0.379	0.069	5.881
Rmeas	0.384	0.070	5.959
Rpim	0.060	0.012	0.951
Total number of observations	1772720	21036	176902
Number of reflections	44627	599	4585
<I/σ(I)>	10.3	48.7	1
Completeness [%]	99.2	60	99.8
Redundancy	39.7	35.1	38.6
CC(1/2)	0.999	0.999	0.455

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	294	3% (w/v) PEG 4,000; 0.15 M sodium acetate; 0.1 M sodium citrate