7MNL
Crystal structure of the N-terminal domain of NUP358/RanBP2 (residues 1-752) in complex with Fab fragment
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-10-09 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0331 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 161.420, 161.420, 644.723 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 19.970 | 19.970 | 4.100 |
High resolution limit [Å] | 3.950 | 14.770 | 3.950 |
Rmerge | 0.379 | 0.069 | 5.881 |
Rmeas | 0.384 | 0.070 | 5.959 |
Rpim | 0.060 | 0.012 | 0.951 |
Total number of observations | 1772720 | 21036 | 176902 |
Number of reflections | 44627 | 599 | 4585 |
<I/σ(I)> | 10.3 | 48.7 | 1 |
Completeness [%] | 99.2 | 60 | 99.8 |
Redundancy | 39.7 | 35.1 | 38.6 |
CC(1/2) | 0.999 | 0.999 | 0.455 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 294 | 3% (w/v) PEG 4,000; 0.15 M sodium acetate; 0.1 M sodium citrate |