7MNK

Crystal structure of the tetramerization element of NUP358/RanBP2 (residues 805-832)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-04-20
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.900
Spacegroup name	I 4 2 2
Unit cell lengths	99.930, 99.930, 60.160
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.140 - 1.100
R-factor	0.155
Rwork	0.155
R-free	0.16690
Structure solution method	SAD
Data reduction software	xia2
Data scaling software	XSCALE
Phasing software	AutoSol
Refinement software	PHENIX (1.18.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.140	29.150	1.140
High resolution limit [Å]	1.100	2.370	1.100
Rmeas	0.063	0.032	3.072
Rpim	0.012	0.006	0.620
Total number of observations	1558137	153904	144840
Number of reflections	61560	6448	6077
<I/σ(I)>	24.1	83.6	1.1
Completeness [%]	100.0	99.9	99.9
Redundancy	25.3	23.9	23.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	3.5	294	2 M ammonium sulfate; 0.1 M citric acid