7MNJ

Crystal structure of the N-terminal domain of NUP358/RanBP2 (residues 145-673)

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-07-06
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.03320
Spacegroup name	P 43 21 2
Unit cell lengths	133.503, 133.503, 286.868
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.850 - 3.800
R-factor	0.2098
Rwork	0.208
R-free	0.24210
Structure solution method	MIRAS
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	autoSHARP
Refinement software	PHENIX (1.18.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.850	29.850	3.940
High resolution limit [Å]	3.800	8.140	3.800
Rmeas	0.089	0.040	1.537
Rpim	0.018	0.008	0.313
Total number of observations	654682	68498	60258
Number of reflections	26310	2843	2545
<I/σ(I)>	18.3	69.8	2.1
Completeness [%]	100.0	100	98.8
Redundancy	24.9	24.1	23.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	294	1% (w/v) PEG 2,000 MME; 0.8 M succinic acid; 0.1 M HEPES