7MN9

PTP1B 1-284 F225Y-R199N

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	.97946
Spacegroup name	P 32 2 1
Unit cell lengths	88.464, 88.464, 72.429
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.310 - 1.240
R-factor	0.1631
Rwork	0.162
R-free	0.18010
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5k9v
RMSD bond length	0.016
RMSD bond angle	1.494
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX (1.18.2_3874)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.310	1.260
High resolution limit [Å]	1.240	1.240
Rmerge	0.056	0.607
Number of reflections	92834	4211
<I/σ(I)>	15.9	1.6
Completeness [%]	99.5	92.1
Redundancy	8.1
CC(1/2)	0.994	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.2 M MgCl2, .1 M Tris pH 7.6, 18.5% PEG